In vitro cleavage of double- and single-stranded DNA by plasmid RSF1010-encoded mobilization proteins.

We have used purified RSF1010 mobilization proteins to reproduce in vitro a strand-specific nicking at the plasmid origin of transfer, oriT. In the presence of Mg2+, the proteins MobA (78-kDa form of RSF1010 DNA primase), MobB, and MobC and supercoiled or linear duplex oriT DNA form large amounts of...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Scherzinger, E, Lurz, R, Otto, S, Dobrinski, B
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 1992
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310323/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1738602/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.1.41
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