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In vitro cleavage of double- and single-stranded DNA by plasmid RSF1010-encoded mobilization proteins.
We have used purified RSF1010 mobilization proteins to reproduce in vitro a strand-specific nicking at the plasmid origin of transfer, oriT. In the presence of Mg2+, the proteins MobA (78-kDa form of RSF1010 DNA primase), MobB, and MobC and supercoiled or linear duplex oriT DNA form large amounts of...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1992
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310323/ https://ncbi.nlm.nih.gov/pubmed/1738602 |
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