Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18

We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and...

Description complète

Détails bibliographiques

Publié dans:	EMBO J
Auteurs principaux:	Kearsey, Stephen E., Montgomery, Stuart, Labib, Karim, Lindner, Karola
Format:	Artigo
Langue:	Inglês
Publié:	Nature Publishing Group 2000
Sujets:	Articles
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10747035/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.7.1681
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!