Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18

We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and...

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Veröffentlicht in:	EMBO J
Hauptverfasser:	Kearsey, Stephen E., Montgomery, Stuart, Labib, Karim, Lindner, Karola
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Nature Publishing Group 2000
Schlagworte:	Articles
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10747035/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.7.1681
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