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Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18
We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310236/ https://ncbi.nlm.nih.gov/pubmed/10747035 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.7.1681 |
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