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Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18

We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and...

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Détails bibliographiques
Auteurs principaux: Kearsey, Stephen E., Montgomery, Stuart, Labib, Karim, Lindner, Karola
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2000
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC310236/
https://ncbi.nlm.nih.gov/pubmed/10747035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.7.1681
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