Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18

We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and...

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Bibliografiska uppgifter

I publikationen:	EMBO J
Huvudupphov:	Kearsey, Stephen E., Montgomery, Stuart, Labib, Karim, Lindner, Karola
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	Nature Publishing Group 2000
Ämnen:	Articles
Länkar:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10747035/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.7.1681
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