Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18

We describe an in situ technique for studying the chromatin binding of proteins in the fission yeast Schizosaccharomyces pombe. After tagging the protein of interest with green fluorescent protein (GFP), chromatin-associated protein is detected by GFP fluorescence following cell permeabilization and...

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Bibliografiske detaljer

Udgivet i:	EMBO J
Principais autores:	Kearsey, Stephen E., Montgomery, Stuart, Labib, Karim, Lindner, Karola
Format:	Artigo
Sprog:	Inglês
Udgivet:	Nature Publishing Group 2000
Fag:	Articles
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10747035/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.7.1681
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