The DNA binding affinity of HhaI methylase is increased by a single amino acid substitution in the catalytic center.
The HhaI methyltransferase recognizes the sequence GCGC and transfers a methyl group to C5 of the first cytosine residue. All m5C-methyltransferases contain a highly conserved sequence motif called the P-C motif. The cysteine residue of this motif is involved in catalysis by forming a covalent bond...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1993
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309547/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8506140/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.10.2459 |
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