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High level expression and purification of HhaI methyltransferase.

A cloning system for the DNA-(cytosine-5)-methyltransferase MHhaI and high level expression of the enzyme are described. A parent plasmid was constructed from fragments of the MHhaI gene and synthetic oligonucleotides. The construct permits introduction of various restriction sites for cloning at pr...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Wu, J C, Santi, D V
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1988
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC334686/
https://ncbi.nlm.nih.gov/pubmed/3340551
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