High level expression and purification of HhaI methyltransferase.
A cloning system for the DNA-(cytosine-5)-methyltransferase MHhaI and high level expression of the enzyme are described. A parent plasmid was constructed from fragments of the MHhaI gene and synthetic oligonucleotides. The construct permits introduction of various restriction sites for cloning at pr...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1988
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334686/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3340551/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.2.703 |
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