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High level expression and purification of HhaI methyltransferase.
A cloning system for the DNA-(cytosine-5)-methyltransferase MHhaI and high level expression of the enzyme are described. A parent plasmid was constructed from fragments of the MHhaI gene and synthetic oligonucleotides. The construct permits introduction of various restriction sites for cloning at pr...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1988
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334686/ https://ncbi.nlm.nih.gov/pubmed/3340551 |
| Tags: |
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