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The DNA binding affinity of HhaI methylase is increased by a single amino acid substitution in the catalytic center.
The HhaI methyltransferase recognizes the sequence GCGC and transfers a methyl group to C5 of the first cytosine residue. All m5C-methyltransferases contain a highly conserved sequence motif called the P-C motif. The cysteine residue of this motif is involved in catalysis by forming a covalent bond...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1993
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309547/ https://ncbi.nlm.nih.gov/pubmed/8506140 |
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