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Site-specific integrase-mediated transgenesis in mice via pronuclear injection

Microinjection of recombinant DNA into zygotic pronuclei has been widely used for producing transgenic mice. However, with this method, the insertion site, integrity, and copy number of the transgene cannot be controlled. Here, we present an integrase-based approach to produce transgenic mice via pr...

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Hlavní autoři: Tasic, Bosiljka, Hippenmeyer, Simon, Wang, Charlene, Gamboa, Matthew, Zong, Hui, Chen-Tsai, Yanru, Luo, Liqun
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3093482/
https://ncbi.nlm.nih.gov/pubmed/21464299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1019507108
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