Загрузка...
Optimized Method for the Synthesis and Purification of Adenosine - Folic Acid Conjugates for use as Transcription Initiators in the Preparation of Modified RNA
We present an optimized synthetic strategy for the attachment of molecules to 5’-adenosine monophosphate (AMP), which can then be used to label the 5'-end of RNA by T7 RNA polymerase mediated in vitro transcription. Through the use of a boronate affinity gel, we have developed an efficient rout...
Сохранить в:
| Главные авторы: | , , |
|---|---|
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2010
|
| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3090705/ https://ncbi.nlm.nih.gov/pubmed/21163352 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.12.007 |
| Метки: |
Добавить метку
Нет меток, Требуется 1-ая метка записи!
|