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Synthesis of adenosine derivatives as transcription initiators and preparation of 5′ fluorescein- and biotin-labeled RNA through one-step in vitro transcription
Expanding our previous finding of an adenosine-initiated transcription system, we now demonstrate that either the 5′ site or the N6 site of adenosine nucleotides can be modified extensively without abolishing their ability to initiate transcription under the T7 φ2.5 promoter. Two series of amino der...
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| Główni autorzy: | , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Copyright 2003 by RNA Society
2003
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370509/ https://ncbi.nlm.nih.gov/pubmed/14624011 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.5106403 |
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