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Quantitative Cell-based Protein Degradation Assays to Identify and Classify Drugs That Target the Ubiquitin-Proteasome System

We have generated a set of dual-reporter human cell lines and devised a chase protocol to quantify proteasomal degradation of a ubiquitin fusion degradation (UFD) substrate, a ubiquitin ligase CRL2(VHL) substrate, and a ubiquitin-independent substrate. Well characterized inhibitors that target diffe...

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Detalles Bibliográficos
Main Authors: Chou, Tsui-Fen, Deshaies, Raymond J.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Biochemistry and Molecular Biology 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3089497/
https://ncbi.nlm.nih.gov/pubmed/21343295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.215319
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