High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation.

We describe a non-isotopic, semi-automated method for large-scale multiplex analysis of nucleic acid sequences, using the cystic fibrosis transmembrane regulator (CFTR) gene as an example. Products of a multiplex oligonucleotide ligation assay (OLA) are resolved electrophoretically from one another...

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Bibliographic Details
Main Authors: Grossman, P D, Bloch, W, Brinson, E, Chang, C C, Eggerding, F A, Fung, S, Iovannisci, D M, Woo, S, Winn-Deen, E S, Iovannisci, D A
Format: Artigo
Language:Inglês
Published: 1994
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC308489/
https://ncbi.nlm.nih.gov/pubmed/7526344
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