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Ligation with Nucleic Acid Sequence–Based Amplification
This work presents a novel method for detecting nucleic acid targets using a ligation step along with an isothermal, exponential amplification step. We use an engineered ssDNA with two variable regions on the ends, allowing us to design the probe for optimal reaction kinetics and primer binding. Thi...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Investigative Pathology
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3349837/ https://ncbi.nlm.nih.gov/pubmed/22449695 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2012.01.004 |
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