Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses

We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, targeted, in situ RNA identification of single or multiple targets. BOLORAMIS was demonstrated on a range of cell types...

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Publicado en:Nucleic Acids Res
Autores principales: Liu, Songlei, Punthambaker, Sukanya, Iyer, Eswar P R, Ferrante, Thomas, Goodwin, Daniel, Fürth, Daniel, Pawlowski, Andrew C, Jindal, Kunal, Tam, Jenny M, Mifflin, Lauren, Alon, Shahar, Sinha, Anubhav, Wassie, Asmamaw T, Chen, Fei, Cheng, Anne, Willocq, Valerie, Meyer, Katharina, Ling, King-Hwa, Camplisson, Conor K, Kohman, Richie E, Aach, John, Lee, Je Hyuk, Yankner, Bruce A, Boyden, Edward S, Church, George M
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8191787/
https://ncbi.nlm.nih.gov/pubmed/33693773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab120
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