Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds.
We have developed general methods for joining together, via cleavable disulfide bonds, either two unprotected polynucleotides or a polynucleotide and a peptide or protein. To join two oligonucleotides, each is first converted to an adduct in which cystamine is joined to the 5'-terminal phosphate of...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1988
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336549/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3375070/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.9.3671 |
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