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Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds.
We have developed general methods for joining together, via cleavable disulfide bonds, either two unprotected polynucleotides or a polynucleotide and a peptide or protein. To join two oligonucleotides, each is first converted to an adduct in which cystamine is joined to the 5'-terminal phosphat...
Αποθηκεύτηκε σε:
| Κύριοι συγγραφείς: | , |
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| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
1988
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| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC336549/ https://ncbi.nlm.nih.gov/pubmed/3375070 |
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