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Substrate Binding and Reactivity are Not Linked: Grafting a Proton-Transfer Network into a Class 1A Dihydroorotate Dehydrogenase

Adding the two residues comprising the conserved proton-transfer network of Class 2 dihydroorotate dehydrogenase (DHOD) to the Cys130Ser Class 1A DHOD did not restore the function of the active site base or rapid flavin reduction. Studies of triple, double, and single Class 1A mutant enzymes showed...

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Bibliografiske detaljer
Main Authors: McDonald, Claudia A., Palfey, Bruce A.
Format: Artigo
Sprog:Inglês
Udgivet: 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3077728/
https://ncbi.nlm.nih.gov/pubmed/21401078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi200258y
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