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Single-molecule kinetics reveals signatures of half-sites reactivity in dihydroorotate dehydrogenase A catalysis

Subunit activity and cooperativity of a homodimeric flavoenzyme, dihydroorotate dehydrogenase A (DHODA) from Lactococcus lactis, were characterized by employing single-molecule spectroscopy to follow the turnover kinetics of individual DHODA molecules, eliminating ensemble averaging. Because the enz...

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書誌詳細
主要な著者: Shi, Jue, Dertouzos, Joe, Gafni, Ari, Steel, Duncan, Palfey, Bruce A.
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 2006
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オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1458649/
https://ncbi.nlm.nih.gov/pubmed/16585513
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0510482103
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