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Turning a protein kinase on or off from a single allosteric site via disulfide trapping

There is significant interest in identifying and characterizing allosteric sites in enzymes such as protein kinases both for understanding allosteric mechanisms as well as for drug discovery. Here, we apply a site-directed technology, disulfide trapping, to interrogate structurally and functionally...

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Autors principals: Sadowsky, Jack D., Burlingame, Mark A., Wolan, Dennis W., McClendon, Christopher L., Jacobson, Matthew P., Wells, James A.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076885/
https://ncbi.nlm.nih.gov/pubmed/21430264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1102376108
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