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Double stranded scission of DNA directed through sequence-specific R-loop formation.
R-loop formation with short (100 nt) RNAs provides a highly flexible and stringent method to achieve sequence-specific separation of target DNA at any given sequence. After stabilization of R-loops with glyoxal and removal of the RNA through RNase treatment the remaining single-stranded DNA bubble p...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1995
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| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307233/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7567465/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.17.3524 |
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