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Double stranded scission of DNA directed through sequence-specific R-loop formation.

R-loop formation with short (100 nt) RNAs provides a highly flexible and stringent method to achieve sequence-specific separation of target DNA at any given sequence. After stabilization of R-loops with glyoxal and removal of the RNA through RNase treatment the remaining single-stranded DNA bubble p...

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Bibliografiske detaljer
Main Authors: Landgraf, R, Chen, C H, Sigman, D S
Format: Artigo
Sprog:Inglês
Udgivet: 1995
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC307233/
https://ncbi.nlm.nih.gov/pubmed/7567465
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