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Double stranded scission of DNA directed through sequence-specific R-loop formation.
R-loop formation with short (100 nt) RNAs provides a highly flexible and stringent method to achieve sequence-specific separation of target DNA at any given sequence. After stabilization of R-loops with glyoxal and removal of the RNA through RNase treatment the remaining single-stranded DNA bubble p...
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| Huvudupphovsmän: | , , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1995
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307233/ https://ncbi.nlm.nih.gov/pubmed/7567465 |
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