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Bacteriophage C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels

Bacteriophage ϕC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integrase requires no accessory factors, has a high efficiency of recombination, and does not ne...

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Detalhes bibliográficos
Main Authors: Allen, Bryan G., Weeks, Daniel L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3071032/
https://ncbi.nlm.nih.gov/pubmed/19085128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-202-1_9
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