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Using ΦC31 integrase to mediate insertion of DNA in Xenopus Embryos

The two most common methods used to generate transgenic Xenopus embryos, restriction enzyme mediated insertion and I-SceI meganuclease, take advantage of relatively common but spatially unpredictable double stranded breaks in sperm, egg or early embryo genomes. These methods also tend to insert mult...

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Bibliografische gegevens
Hoofdauteurs: Li, You E., Allen, Bryan G., Weeks, Daniel L.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2012
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3551469/
https://ncbi.nlm.nih.gov/pubmed/22956091
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-992-1_13
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