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Using ΦC31 integrase to mediate insertion of DNA in Xenopus Embryos
The two most common methods used to generate transgenic Xenopus embryos, restriction enzyme mediated insertion and I-SceI meganuclease, take advantage of relatively common but spatially unpredictable double stranded breaks in sperm, egg or early embryo genomes. These methods also tend to insert mult...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3551469/ https://ncbi.nlm.nih.gov/pubmed/22956091 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-992-1_13 |
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