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Transgenic Xenopus laevis embryos can be generated using ϕC31 integrase

Phage ϕC31 encodes an integrase that can mediate the insertion of extrachromosomal DNA into genomic DNA(1). Here we show ϕC31 integrase can be used to generate transgenic Xenopus laevis embryos. mRNA encoding integrase was co-injected with a reporter plasmid containing a CMV promoter driven GFP into...

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Detalles Bibliográficos
Main Authors: Allen, Bryan G., Weeks, Daniel L.
Formato: Artigo
Idioma:Inglês
Publicado: 2005
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3552317/
https://ncbi.nlm.nih.gov/pubmed/16299484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth814
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