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Transgenic Xenopus laevis embryos can be generated using ϕC31 integrase
Phage ϕC31 encodes an integrase that can mediate the insertion of extrachromosomal DNA into genomic DNA(1). Here we show ϕC31 integrase can be used to generate transgenic Xenopus laevis embryos. mRNA encoding integrase was co-injected with a reporter plasmid containing a CMV promoter driven GFP into...
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Main Authors: | , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2005
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3552317/ https://ncbi.nlm.nih.gov/pubmed/16299484 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth814 |
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