Iterative in vivo assembly of large and complex transgenes by combining the activities of φC31 integrase and Cre recombinase

We have used the φC31 integrase to introduce large DNA sequences into a vertebrate genome and measure the efficiency of integration of intact DNA as a function of insert size. Inserts of 110 kb and 140 kb in length may be integrated with about 25% and 10% efficiency respectively. In order to overcom...

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Bibliographic Details
Main Authors: Dafhnis-Calas, Felix, Xu, Zhengyao, Haines, Steve, Malla, Sunir K., Smith, Margaret C. M., Brown, William R. A.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2005
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1316120/
https://ncbi.nlm.nih.gov/pubmed/16361264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni192
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