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Iterative in vivo assembly of large and complex transgenes by combining the activities of φC31 integrase and Cre recombinase
We have used the φC31 integrase to introduce large DNA sequences into a vertebrate genome and measure the efficiency of integration of intact DNA as a function of insert size. Inserts of 110 kb and 140 kb in length may be integrated with about 25% and 10% efficiency respectively. In order to overcom...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2005
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1316120/ https://ncbi.nlm.nih.gov/pubmed/16361264 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni192 |
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