Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage ϕBT1

We have established the integrase of the Streptomyces phage ϕBT1 as a tool for eukaryotic genome manipulation. We show that the ϕBT1 integrase promotes efficient reciprocal and conservative site-specific recombination in vertebrate cells and in Schizosaccharomyces pombe, thus establishing the utilit...

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Detalles Bibliográficos
Main Authors: Xu, Zhengyao, Lee, Nicholas C. O., Dafhnis-Calas, Felix, Malla, Sunir, Smith, Margaret C. M., Brown, William R. A.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2008
Assuntos:
Methods Online
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2248738/
https://ncbi.nlm.nih.gov/pubmed/18096621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm1123
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