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Effective RNAi-mediated β2-microglobulin loss of function by transgenesis in Xenopus laevis
To impair MHC class I (class I) function in vivo in the amphibian Xenopus, we developed an effective reverse genetic loss of function approach by combining I-SceI meganuclease-mediated transgenesis with RNAi technology. We generated transgenic outbred X. laevis and isogenetic laevis/gilli cloned lin...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Company of Biologists
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3603415/ https://ncbi.nlm.nih.gov/pubmed/23519478 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.20133483 |
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