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Electrophoresis for genotyping: temporal thermal gradient gel electrophoresis for profiling of oligonucleotide dissociation.
Traditional use of an oligonucleotide probe to determine genotype depends on perfect base pairing to a single-stranded target which is stable to a higher temperature than when imperfect binding occurs due to a mismatch in the target sequence. Bound oligonucleotide is detected at a predetermined sing...
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| Main Authors: | , , , , |
|---|---|
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
1995
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| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307044/ https://ncbi.nlm.nih.gov/pubmed/7630718 |
| Clibeanna: |
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