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Electrophoresis for genotyping: temporal thermal gradient gel electrophoresis for profiling of oligonucleotide dissociation.

Traditional use of an oligonucleotide probe to determine genotype depends on perfect base pairing to a single-stranded target which is stable to a higher temperature than when imperfect binding occurs due to a mismatch in the target sequence. Bound oligonucleotide is detected at a predetermined sing...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Autores principales: Day, I N, O'Dell, S D, Cash, I D, Humphries, S E, Weavind, G P
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 1995
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307044/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7630718/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.13.2404
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