Electrophoresis for genotyping: temporal thermal gradient gel electrophoresis for profiling of oligonucleotide dissociation.

Traditional use of an oligonucleotide probe to determine genotype depends on perfect base pairing to a single-stranded target which is stable to a higher temperature than when imperfect binding occurs due to a mismatch in the target sequence. Bound oligonucleotide is detected at a predetermined sing...

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Bibliografiske detaljer
Main Authors: Day, I N, O'Dell, S D, Cash, I D, Humphries, S E, Weavind, G P
Format: Artigo
Sprog:Inglês
Udgivet: 1995
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC307044/
https://ncbi.nlm.nih.gov/pubmed/7630718
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