Electrophoresis for genotyping: temporal thermal gradient gel electrophoresis for profiling of oligonucleotide dissociation.
Traditional use of an oligonucleotide probe to determine genotype depends on perfect base pairing to a single-stranded target which is stable to a higher temperature than when imperfect binding occurs due to a mismatch in the target sequence. Bound oligonucleotide is detected at a predetermined sing...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1995
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307044/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7630718/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.13.2404 |
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