Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system.

The FLP protein, a site-specific recombinase encoded by the 2 micron plasmid of yeast, has been purified to near homogeneity from extracts of E. coli cells in which the protein has been expressed. The purification is a three column procedure, the final step employing affinity chromatography. The aff...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Meyer-Leon, L, Gates, C A, Attwood, J M, Wood, E A, Cox, M M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1987
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306117/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3306602/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.16.6469
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