Convenient and reversible site-specific targeting of exogenous DNA into a bacterial chromosome by use of the FLP recombinase: the FLIRT system.

We have created a system that utilizes the FLP recombinase of yeast to introduce exogenous cloned DNA reversibly at defined locations in the Escherichia coli chromosome. Recombination target (FRT) sites can be introduced permanently at random locations in the chromosome on a modified Tn5 transposon,...

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Bibliographic Details

Published in:	J Bacteriol
Main Authors:	Huang, L C, Wood, E A, Cox, M M
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 1997
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC179511/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9324255/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.19.6076-6083.1997
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