Convenient and reversible site-specific targeting of exogenous DNA into a bacterial chromosome by use of the FLP recombinase: the FLIRT system.
We have created a system that utilizes the FLP recombinase of yeast to introduce exogenous cloned DNA reversibly at defined locations in the Escherichia coli chromosome. Recombination target (FRT) sites can be introduced permanently at random locations in the chromosome on a modified Tn5 transposon,...
Zapisane w:
| Wydane w: | J Bacteriol |
|---|---|
| Główni autorzy: | , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Microbiology (ASM)
1997
|
| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC179511/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9324255/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.19.6076-6083.1997 |
| Etykiety: |
Nie ma etykietki, Dołącz pierwszą etykiete!
|