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Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system.
The FLP protein, a site-specific recombinase encoded by the 2 micron plasmid of yeast, has been purified to near homogeneity from extracts of E. coli cells in which the protein has been expressed. The purification is a three column procedure, the final step employing affinity chromatography. The aff...
সংরক্ষণ করুন:
| প্রধান লেখক: | , , , , |
|---|---|
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
1987
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| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306117/ https://ncbi.nlm.nih.gov/pubmed/3306602 |
| ট্যাগগুলো: |
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