Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system.
The FLP protein, a site-specific recombinase encoded by the 2 micron plasmid of yeast, has been purified to near homogeneity from extracts of E. coli cells in which the protein has been expressed. The purification is a three column procedure, the final step employing affinity chromatography. The aff...
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| Vydáno v: | Nucleic Acids Res |
|---|---|
| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1987
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306117/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3306602/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.16.6469 |
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