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Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system.

The FLP protein, a site-specific recombinase encoded by the 2 micron plasmid of yeast, has been purified to near homogeneity from extracts of E. coli cells in which the protein has been expressed. The purification is a three column procedure, the final step employing affinity chromatography. The aff...

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Bibliografiske detaljer
Main Authors: Meyer-Leon, L, Gates, C A, Attwood, J M, Wood, E A, Cox, M M
Format: Artigo
Sprog:Inglês
Udgivet: 1987
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC306117/
https://ncbi.nlm.nih.gov/pubmed/3306602
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