Direct method for detecting small quantities of hepatitis B virus DNA in serum and plasma using the polymerase chain reaction.

Serum components inhibit DNA polymerase, thereby obviating direct detection of serum viral DNA sequences by the polymerase chain reaction (PCR). This has necessitated extraction of nucleic acid from sera before performing PCR and has resulted in loss of sensitivity. By adsorbing virus to a solid sur...

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Detalles Bibliográficos
Publicado en:J Clin Invest
Main Authors: Zeldis, J B, Lee, J H, Mamish, D, Finegold, D J, Sircar, R, Ling, Q, Knudsen, P J, Kuramoto, I K, Mimms, L T
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Clinical Investigation 1989
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC304015/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2808703/
https://ncbi.nlm.nih.govhttps://doi.org/10.1172/JCI114326
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