Rapid and sensitive method for the detection of serum hepatitis B virus DNA using the polymerase chain reaction technique.

We have developed a rapid procedure for the detection of serum hepatitis B virus (HBV) DNA using the polymerase chain reaction (PCR) technique. HBV DNA is released from virions by incubating serum with 0.1 M NaOH for 60 min at 37 degrees C. The mixture is brought to neutral pH with HCl, and the HBV...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Kaneko, S, Feinstone, S M, Miller, R H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1989
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267711/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2778059/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.27.9.1930-1933.1989
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