Direct method for detecting small quantities of hepatitis B virus DNA in serum and plasma using the polymerase chain reaction.

Serum components inhibit DNA polymerase, thereby obviating direct detection of serum viral DNA sequences by the polymerase chain reaction (PCR). This has necessitated extraction of nucleic acid from sera before performing PCR and has resulted in loss of sensitivity. By adsorbing virus to a solid sur...

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Dettagli Bibliografici
Pubblicato in:J Clin Invest
Autori principali: Zeldis, J B, Lee, J H, Mamish, D, Finegold, D J, Sircar, R, Ling, Q, Knudsen, P J, Kuramoto, I K, Mimms, L T
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Clinical Investigation 1989
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Research Article
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC304015/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2808703/
https://ncbi.nlm.nih.govhttps://doi.org/10.1172/JCI114326
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