Stabilization of the Single-Chain Fragment Variable by an Interdomain Disulfide Bond and Its Effect on Antibody Affinity

The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4—an anti-aflatoxin B(1) (AFB(1)) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibod...

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Detalhes bibliográficos
Main Authors: Zhao, Jian-Xin, Yang, Lian, Gu, Zhen-Nan, Chen, Hai-Qin, Tian, Feng-Wei, Chen, Yong-Quan, Zhang, Hao, Chen, Wei
Formato: Artigo
Idioma:Inglês
Publicado em: Molecular Diversity Preservation International (MDPI) 2010
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3039938/
https://ncbi.nlm.nih.gov/pubmed/21339972
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms12010001
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