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Mycobacterial Ubiquitin-like Protein Ligase PafA Follows a Two-step Reaction Pathway with a Phosphorylated Pup Intermediate

In Mycobacterium tuberculosis, the enzyme PafA is responsible for the activation and conjugation of the proteasome-targeting molecule Pup to protein substrates. As the proteasomal pathway has been shown to be vital to the persistence of M. tuberculosis, understanding the reaction mechanism of PafA i...

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Bibliographic Details
Main Authors: Guth, Ethan, Thommen, Michael, Weber-Ban, Eilika
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2011
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3039397/
https://ncbi.nlm.nih.gov/pubmed/21081505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.189282
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