Production of active recombinant eIF5A: reconstitution in E.coli of eukaryotic hypusine modification of eIF5A by its coexpression with modifying enzymes

Eukaryotic translation initiation factor 5A (eIF5A) is the only cellular protein that contains the polyamine-modified lysine, hypusine [N(ε)-(4-amino-2-hydroxybutyl)lysine]. Hypusine occurs only in eukaryotes and certain archaea, but not in eubacteria. It is formed post-translationally by two consec...

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Hlavní autoři: Park, Jong Hwan, Dias, Camila A. O., Lee, Seung Bum, Valentini, Sandro R., Sokabe, Masaaki, Fraser, Christopher S., Park, Myung Hee
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2011
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Orginal Articles
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3038461/
https://ncbi.nlm.nih.gov/pubmed/21131325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzq110
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