Pressurized Pepsin Digestion in Proteomics: AN AUTOMATABLE ALTERNATIVE TO TRYPSIN FOR INTEGRATED TOP-DOWN BOTTOM-UP PROTEOMICS*

Integrated top-down bottom-up proteomics combined with on-line digestion has great potential to improve the characterization of protein isoforms in biological systems and is amendable to high throughput proteomics experiments. Bottom-up proteomics ultimately provides the peptide sequences derived fr...

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Bibliographische Detailangaben
Hauptverfasser: López-Ferrer, Daniel, Petritis, Konstantinos, Robinson, Errol W., Hixson, Kim K., Tian, Zhixin, Lee, Jung Hwa, Lee, Sang-Won, Tolić, Nikola, Weitz, Karl K., Belov, Mikhail E., Smith, Richard D., Paša-Tolić, Ljiljana
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2011
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Special Issue
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3033671/
https://ncbi.nlm.nih.gov/pubmed/20627868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M110.001479
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