An integrated top-down and bottom-up strategy for broadly characterizing protein isoforms and modifications

We present an integrated top-down and bottom-up approach that is facilitated by concurrent liquid chromatography-mass spectrometry (LC-MS) analysis and fraction collection for comprehensive high-throughput intact protein profiling. The approach employs high resolution reversed phase (RP) LC separati...

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Bibliografiske detaljer
Main Authors: Wu, Si, Lourette, Natacha M., Tolić, Nikola, Zhao, Rui, Robinson, Errol W., Tolmachev, Aleksey V., Smith, Richard D., Paša-Tolić, Ljiljana
Format: Artigo
Sprog:Inglês
Udgivet: 2009
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2693490/
https://ncbi.nlm.nih.gov/pubmed/19206473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr800720d
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