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Application of pressurized solvents for ultra fast trypsin hydrolysis in proteomics: Proteomics on the fly
A new method for rapid proteolytic digestion of proteins under high pressure that uses pressure cycling technology in the range of 5 to 35 kpsi was demonstrated for proteomic analysis. Successful in-solution digestions of single proteins and complex protein mixtures were achieved in 60 s and then an...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2744211/ https://ncbi.nlm.nih.gov/pubmed/18605748 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr7008077 |
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