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Evaluation of a High Intensity Focused Ultrasound-Immobilized Trypsin Digestion and (18)O-Labeling Method for Quantitative Proteomics

A new method that uses immobilized trypsin concomitant with ultrasonic irradiation results in ultra-rapid digestion and thorough (18)O labeling for quantitative protein comparisons. The reproducible and highly efficient method provided effective digestions in <1 min with a minimized amount of enz...

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Bibliografiska uppgifter
Huvudupphovsmän: López-Ferrer, Daniel, Hixson, Kim K., Smallwood, Heather, Squier, Thomas C., Petritis, Konstantinos, Smith, Richard D.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2009
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2765551/
https://ncbi.nlm.nih.gov/pubmed/19555078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac802540s
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